In this course students learn proper lab protocols and techniques from a life-science industry lab specialist. Students learn by performing hands-on preparation protocols and wet lab techniques. The course covers a variety of categories but begins with safety and the proper use of lab equipment from mass balances and centrifuges to water baths and pipettes.
After students become completely familiar with the proper operation of lab equipment and the centrality of lab safety, they will perform lab experiments in some key life science areas to demonstrate the results of learned protocols and techniques. They will have an opportunity to explore meaning and applications.
Students will explore molecular biology focusing upon the foundations of molecular cloning which includes a set of techniques used to insert recombinant DNA from a prokaryotic or eukaryotic source into a replicating vehicle such as plasmids or viral vectors. Students will learn about the different steps of molecular cloning, how to set up the procedures as well as the different applications of each of the technique, which include:
- Plasmid Minipreps
- Polymerase Chain Reaction
Another area for exploration will include proteins. From the wet-lab perspective, experiments to be explored will include:
- Protein Gel Electrophoresis
- Western Blotting
Students will also investigate cell biology from the wet-lab perspective and will engage in experiments that help them to learn about:
- Tissue culture
By course end, students will be confident and competent in an important range of frequently used wet-lab protocols and the techniques that increase the probability of successful experimentation in a wet-lab.